Reactive oxygen species and antioxidants relationship in the internal spermatic vein blood of infertile men with varicocele.
نویسندگان
چکیده
AIM To assess the relation of reactive oxygen species (ROS) and antioxidants in the internal spermatic vein blood compared to the peripheral venous blood. METHODS Sixty-eight infertile oligoasthenozoospemic patients associated with varicocele were investigated. During inguinal varicocelectomy, blood samples of internal spermatic as well as median cubital veins were withdrawn. Three ROS factors (malondialdehyde [MDA], hydrogen peroxide H(2)O(2), nitric oxide [NO]) and four antioxidants (superoxide dismutase [SOD], catalase [Cat], glutathione peroxidase [GPx] and vitamin C) were estimated in these blood samples. RESULTS Mean levels of tested ROS factors were significantly higher in the internal spermatic venous blood compared to those in the peripheral one (mean+/-SD) (MDA 18.7+/-1.4 nmol/mL vs. 15.4+/-1.4 nmol/mL, H(2)O(2) 43.6+/-8.0 micromol/mL vs. 30.8+/-8.1 micromol/mL, NO 2.3+/-0.5 nmol/L vs. 1.6+/-0.4 nmol/L, P<0.01). Mean levels of tested antioxidants were significantly lower in the internal spermatic venous blood compared to those in the peripheral one (superoxide dismutase 1 690.7+/-130.0 U/mL vs. 1 818.5+/-143.0 U/mL, catalase 38.9+/-6.1 mol/L vs. 47.9+/-10.2 mol/L, GPx 20.4+/-8.1 U/mL vs. 23.0+/-8.4 U/mL, vitamin C 0.3+/-0.1 vs. 0.4+/-0.1 mg/dL, P<0.05). CONCLUSION Internal spermatic venous blood of infertile male cases associated with varicocele demonstrated elevated levels of ROS and decreased levels of antioxidants compared to peripheral venous circulation.
منابع مشابه
Reactive Oxygen Species in the Internal Spermatic and Brachial Veins of Patients with Varicocele-Induced Infertility
PURPOSE The aim of this study was to evaluate the relationship between the levels of reactive oxygen species (ROS) and the clinical characteristics of varicoceles among patients with varicocele-induced infertility. MATERIALS AND METHODS Ninety-eight patients with infertile varicocele and 22 control subjects without varicocele were enrolled. Blood samples were drawn from the brachial vein (BV)...
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Background Varicocele is dilatation of the veins of the pampiniform plexus within the spermatic cord and is one of the amendable causes of male infertility. Many studies have revealed increase of oxidative stress in serum, semen and testicular tissues of patients with varicocele which may cause DNA damage. HSP70 are a family of proteins that can be expressed in response to a variety of stress, ...
متن کاملP-114: Evaluation of GSTM1 Null Allele in Infertile Men with Varicocele
Background Varicocele is the abnormal inflexion and distension of veins of the pampiniform plexus within spermatic cord and is the most common and correctable known risk factor for male infertility. Varicoceles are found in approximately 40% of infertile men, whereas the incidence in the general male population is approximately 15%. Some studies suggest that varicocele is associated with elevat...
متن کاملMitochondrial Genetic Variation in Iranian Infertile Men with Varicocele
Objective Several recent studies have shown that mitochondrial DNA mutations lead to major disabilities and premature death in carriers. More than 150 mutations in human mitochondrial DNA (mtDNA) genes have been associated with a wide spectrum of disorders. Varicocele, one of the causes of infertility in men wherein abnormal inflexion and distension of veins of the pampiniform plexus is observe...
متن کاملVaricocele is associated with elevated spermatozoal reactive oxygen species production and diminished seminal plasma antioxidant capacity.
PURPOSE Because varicocele is seen often in infertile men and oxidative stress has been implicated in sperm dysfunction, we assessed spermatozoal reactive oxygen species and seminal total antioxidant capacity in men with and without varicocele. MATERIALS AND METHODS Levels of reactive oxygen species and total antioxidant capacity were measured in the semen of 21 infertile men with varicocele,...
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ورودعنوان ژورنال:
- Asian journal of andrology
دوره 8 4 شماره
صفحات -
تاریخ انتشار 2006